Laboratory Research Technician III

The candidate will join the Mouse Embryo Services (MES) Core, within the Department of Immunology. Mouse Embryo Services (MES) Core provides a broad, comprehensive range of services to University of Pittsburgh investigators. The individual will be involved in all services in MES core, including generation of gene editing mouse models, cryopreservation, recovery of mouse lines from cryopreserved germplasm and long-term storage of mouse sperm/embryos. Individuals will be cross trained to provide assistance as necessary to other projects in the Core laboratory.

Job Summary

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Essential Functions

Duties include and not limited:

1. 1. Mouse surgery including embryo transfer, vasectomy male surgery 2. Manage mouse colony. Duties include mouse strain breeding, collecting samples for genotyping, weaning et al. 3. Re-derive mouse from cryopreserved sperm and embryos. 4. Mouse sperm/embryo cryopreservation. 5. Generation of gene modified mouse lines, performing mouse pronuclear microinjection and cytoplasmic injection 6. Cryo-preserved samples shipment 7. Maintenance of timely communication with the primary investigator (PI) to provide updates on all scientific projects.

Physical Effort

Minimal Physical Effort Required

The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.

The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.

Assignment Category: Full-time regular Job Classification: Staff.Laboratory Research Technician III Job Family: Research Job Sub Family: Laboratory Campus: Pittsburgh Minimum Education Level Required: Bachelors Degree Minimum Years of Experience Required: 2 Will this position accept substitution in lieu of education or experience: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement. Work Schedule: Monday - Friday, 8:30 a.m. - 5:00 p.m. Work Arrangement: Monday - Friday, 8:30 a.m. - 5:00 p.m. Hiring Range: $37,183.00 to $55,773.00 Relocation_Offered: No Visa Sponsorship Provided: No Background Check: For position finalists, employment with the University will require successful completion of a background check Child Protection Clearances: Not Applicable Required Documents: Resume Optional Documents: Cover Letter PI245001717