Core Research Facility Technician III - NextGen DNA Sequencing

Apply now (https://secure.dc4.pageuppeople.com/apply/674/gateway/default.aspx?c=apply&lJobID=532251&lJobSourceTypeID=796&sLanguage=en-us) Job no: 532251

Work type: Staff Full-Time

Location: Main Campus (Gainesville, FL)

Categories: Biology/Life Science

Department: 11600107 - RE-ICBR-NEXTGEN

Classification Title:

Core Research Facility Technician III

Job Description:

Technical Functions:

-Plan and execute Illumina MiSeq sequencing runs.

-Maintain and troubleshoot Illumina MiSeq instruments.

-Evaluate MiSeq, iSeq and ONT instruments data and prepare written reports for research projects.

-Construct and perform quality assessment of samples, and sequence libraries for the ONT, PacBio and Illumina platforms.

-Test, troubleshoot and implement new protocols for ultra-low input (Illumina) library construction protocol, using novel procedures as they become available in the literature or from vendors.

-Communicate with supervisor and other core personnel about experimental results in a timely and professional manner. Work with supervisor to define deliverables and timelines for project completion.

-Contribute to the preparation, updating and organization of Standard Operating Procedures (SOPs).

General Lab Operation and Maintenance Functions:

-Contribute to the development and maintenance of lab tracking system (LIMS) in the NGS workflows.

-Attend all relevant coordination and organizational meetings as requested by supervisor.

-Participate in general lab maintenance, help in the maintenance of a complete inventory of reagents, consumables and equipment used for NGS sequencing.

-Assist lab manager in keeping track of inventories and placing orders of reagents, consumables for ONT, PacBio and Illumina sequencing as needed.

-Assist the Environmental Health and Safety (EHS) manager for the NextGen lab as needed, for the purpose of complying with all EHS institutional requirements.

Educational Functions:

-Keep abreast of new developments in the NGS field through the literature and scientific meetings.

-Help in the training of other personnel in the NGS Core group. Participate in cross-training activities in the ICBR as necessary and/or requested by lab director.

Expected Salary:

$44,000 - $46,000, commensurate with education and experience

Minimum Requirements:

BA/BS in related scientific field required with hands-on experience in basic laboratory and scientific methods. Optionally, 4 years of experience in science related field with at least 3 years in core specific applications.

Core or technology specific licensure and/or certifications where applicable. Additionally yearly certificate and compliance training if required.

Preferred Qualifications:

The preferred applicant will have a familiarity with the basic techniques and instrumentation used in the molecular biology laboratory, including specific NGS procedures that include (but are not limited to):

• Genomic DNA purification, quantification and manipulation, PCR, NGS DNA library construction and quality assessment/evaluation.

• Use of robotics for high throughput processing of moeluclar biology samples.

• Hands-on experience with Illumina, PacBio and/or ONT NGS instrumentation and technology.

• Basic laboratory citizenship including organization of equipment and supply inventory,

• Instrument run coordination and maintenance.

• Preparation of common buffers and solutions.

• Computer literacy in Windows/UNIX systems, Microsoft office package.

• Standard Operating Procedures (SOPs), iLAB and laboratory information management system (LIMS).

Special Instructions to Applicants:

In order to be considered, you must upload your cover letter and resume.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required:No

Advertised: 24 Jul 2024 Eastern Daylight Time

Applications close: 07 Aug 2024 Eastern Daylight Time